gram negative unknown lab report essay sample abstract the unknown gram negative bacterium inoculated in a tryptic soy broth medium was randomly selected from a group of other unknowns. Microbiology lab report for unknown margaret olivero microbiology lab 1290 october 26, 2013 identify the unknown organisms citrobacter freundii, enterobacter aerogenes, escherichia coli, klebsiella pneumonia, proteus vulgaris, salmonella, shigella flexneri. When find an unknown bacterium, you first make a pure culture of it on an agar plate are purple under the microscope and gram-negative escherichia coli and. The microbes that were tested during this lab were: escherichia coli, bacillus cereus, the unknown, proteus vulgaris, staphylococcus epidermis, enterobacter aerogenes, the control, and pseudomonas fluorescens. You should be prepared to turn in your notebook with your biochemical unknown possible unknown bacterial species gram-negative enterobacter aerogenes.
Microbiology: unknown test 1 simple stain unknown shape: bacillus unknown couldn't be 51 organisms 37% eliminated organisms remaining: 87/63% bacillus subtilis, bacillus cereus, erwinia carotovora, bacillus megaterium, listeria monocytogenes, arthrobacter globiformis, citrobacter freundii. Unknown lab report once a gram stain concluded that unknown a was gram negative rods the pure culture was then inoculated onto a mannitol salt agar plate. O gram-negative enterobacteria escherichia coli and enterobacter aerogenes ferment lactose o e coli produces colonies with a characteristic green metallic sheen on emb agar o e aerogenes produces pink colonies often with a central dark purple dot (fish eye colonies) on emb agar. Lab #04 identification information assigned unknown: identified unknown: gram reaction and morphology: gram negative rods enterobacter aerogenes, enterobacter.
According to the results from the data collected, the unknown gram-negative bacterium was identified as a lactose fermenter and therefore the unknown bacterium could be any of the enterobacteriaeae including escherichia coli, enterobacter aerogenes, klebsiella pnuemoniae. Enterobacter cloacae is a clinically significant gram-negative, facultatively-anaerobic, rod-shaped bacterium. For the gram stain, my organism turned red, meaning that my bacterium was a gram negative and it was also rod shaped after this test, the possible organisms were: enterobacter aerogenes, escherichia coli, serratia marcescens, citrobacter freundii, proteus mirabilis, pseudomonas aeruginosa, pseudomonas fluorescens, salmonella or shigella. Enterobacter aerogenes has been plated on several different medias and have been observed under several types of testing the results are as follows- e aerogenes tested negative when treated with/for: indol, methyl red, hydrogen sulfide (by way of tsi), urease, arginine dihydrolase, phenylalanine deaminase, and dulcitol.
After determining the gram reaction purpose since this is lab report for the identification of an unknown bacterium and the methods are explained in detail in the lab manual oxidase test 2 since unknown g was determined to be a gram negative rod the morphology was observed and recorded and a gram stain was performed. Enterobacter species were the second-most-common gram-negative organism behind pseudomonas aeruginosa however, both bacteria were reported to each represent 47% of bloodstream infections in icu settings. Considering that gram-negative bacillus and gram-positive cocci were observed, the following list of tests was performed in order to determine the unknown sample according to the lab manual procedures (choi 2014. Enterobacter aerogenes is a gram-negative rod-shaped bacterium belonging to the enterobacteriacease family it commonly resides in the human gastrointestinal tract and does not cause infections/diseases in healthy people but inthose who have weakened immune system.
The purpose of this lab is to isolate a bacterial population from the normal throat flora - unknown lab report introduction a streak plate method will be used to obtain a pure culture of a gram positive coccus genus of bacteria. Enterobacter aerogenes is a nosocomial and pathogenic bacterium that causes opportunistic infections it is a gram-negative rod shaped bacteria e aerogenes is generally found in the human gastrointestinal tract and does not generally cause disease in healthy individuals. The unknown gram-negative bacteria is enterobacter aerogenes the other bacteria which was microscopically viewed as gram positive was missing in the incubator, another tube of sample was derived from the lab professor.
Unknown lab report unknown organism #6 ann le (phuoc) may 6, 2010 dr carrington microbiology lab- mw 12:50 le 1 i introduction my unknown organism #6 is morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora (3, 5) this. Staphylococcus aureus flowchart 1 unknown 103 unknown a gram positive gram stain gram positive cocci staphylococcus aureus staphylococcus epidermidis enterococcus how to identify pseudomonas aeruginosa unknown lab report sophie fisher microbiology introduction it is quite important to be able to identify various microbes such as bacteria fungi and how to identify bacillus cereus steps to.
Imvic tests of enterobacter aerogenes indole: negative mycobacteria biochemical tests for gram negative bacteria biochemical tests for gram lab diagnosis of. Citrobacter diversus, c freundii, enterobacter aerogenes, e cloacae, erwinia chrysanthemi and differentiate them from other gram-negative rods images from the. Klebsiella aerogenes, previously known as enterobacter aerogenes, is a gram-negative, oxidase negative, catalase positive, citrate positive, indole negative, rod-shaped bacterium the bacterium is approximately 1-3 microns in length, and is capable of motility via peritrichous flagella. Keywords: enterobacter aerogenes, enterobacter cloacae, membrane and transporters, regulation, resistance mechanisms introduction enterobacter is a genus of a common gram-negative, facultative anaerobic, rod-shaped, non-spore-forming bacteria belonging to the family enterobacteriaceae.